HIV Reservoir characterisation Workshop: 14-16 september 2018
Challenges and ethical considerations of HIV cure and treatment interruption trials will be thoroughly discussed during this year’s workshops. Moreover, workshops will provide the participants with a thorough understanding of HIV quantification tools including PCR-based and cell-based functional assays, which are of great importance in therapy monitoring for the development of HIV cure strategies. An introductory course to ex vivo viral outgrowth assays and its use to quantify the replication competent HIV reservoir will be covered. The participants will receive an intensive training on PCR, qPCR, digital and digital droplet PCR methods and single genome sequencing with a comprehensive overview of clinical use of these methods and clinical significance of various HIV markers. Novel aspects of HIV persistence such as clonal expansion and integration site analysis will also be discussed and thorough overview of assays to monitor them will be presented. Given that the host’s immune system is an indispensable component of an effective cure strategy, in addition to virological assays, the workshop will present an overview of immunological assays that are commonly used to assess the competency of the immune system. The workshop will conclude with comprehensive hands-on sessions that will include statistical data analysis training sessions.
Researchers interested in: low level RNA or DNA quantification by digital PCR and immune monitoring assays especially in the field of HIV research.
Friday 14 September
Introduction to digital PCR and hands-on training
|08:30-08:40||Linos Vandekerckhove||HIV Cure Research Center UGent||Welcome|
|08:40-09:40||Ward De Spiegelaere||Ghent University||Description of PCR based methods: qPCR and digital PCR|
|10:00-12:30||Practical exercises dPCR||Hands on practice dPCR platform|
|13:30-15:45||Practical exercises dPCR||Hands on practice dPCR platform|
|16:00-16:30||Q&A practical exercises dPCR|
|16:30-17:30||Clarissa Van Hecke and Sofie Rutsaert||HIV Cure Research Center UGent||qPCR and droplet digital PCR data analysis in practice.|
|09:00-09:30||Linos Vandekerckhove||HIV Cure Research Center UGent||Introduction in HIV-1 reservoir characterisation|
|09:30-10:30||Marie-Angelique De Scheerder||HIV Cure Research Center UGent||How to set up HIV Cure studies|
|10:50-12:00||Sofie Rutsaert||HIV Cure Research Center UGent||Clinical applicability and clinical significance of HIV markers (total HIV DNA + Integrated HIV DNA)|
|13:30-14:30||Mathias Lichterfeld||Ragon Institute||Full length HIV sequencing|
|14:30-15:45||Eva Malatinkova and Maja Kiselinova||HIV Cure Research Center UGent||Markers for transcription or ongoing replication: Cell-associated HIV RNA +2LTR quantification|
|16:15-17:15||Marie-Angelique De Scheerder + Basiel Cole||HIV Cure Research Center UGent||Sequencing virus, host and their interface: different approaches?|
Phenotypical and immunological assessment.
|9:00-10:00||Maja Kiselinova||HIV Cure Research Center UGent||VOA & Tilda assays|
|10:00-11:00||Philipp Adams||Infectious Diseases Research Unit, Luxembourg Institute of Health||Humanized Mice model in HIV research|
|11:30-12:30||Susan Pereira Ribeiro||Case Western Reserve University, Pathology Department, Sekaly's Group||Single cell assessment of HIV reservoir: establishment, maintenance and reactivation - in vitro and ex vivo approaches|
|13:30-14:30||Magdalena Sips||HIV Cure Research Center UGent||Monitoring the immune system at the level of cell, tissue and organ.|
|14:45-15:45||Wojciech Witkowski||HIV Cure Research Center UGent||Broadly neutralizing antibodies in HIV-1 immunotherapy.|
|15:45-16:00||Linos Vandekerckhove||HIV Cure Research Center UGent||Conclusion|
Registration for the workshop includes registration for the minisymposium on 13 September 2018. It is possible to register from 01/03/2018 until 31/08/2018 (early bird deadline is 30/06/2018). More information can be found here.
The amount of participants is limited to 30 researchers.
Corneel Heymanslaan 10, 9000 Ghent
By public transport
Participants arriving at Brussels airport can take the train to Ghent Sint Pieters station. Frequent trains are available (http://www.belgianrail.be/en).
From Ghent Sint Pieters Station, take tram 4 direction ‘UZ Gent’. The last stop is in the middle of the University Hospital. At the hospital campus, follow the directions of entrance 38 (MRBII). You can find a map of the campus here.
There is limited parking available on the campus.
The campus is accessible from the highway E40 and E17: E40 (Oostende-Brussel)is connected to E17 (Antwerpen-Kortrijk) in Zwijnaarde. From E17, take exit 9 (‘Universitair Ziekenhuis’ or ‘UZG’).