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Workshop 2017-07-19T10:20:20+00:00

Optional HIV Reservoir characterisation Workshop: 13-15 september

Scope

The workshop will provide the participants with a thorough understanding of HIV quantification tools including PCR-based and cell-based functional assays, which are of great importance in therapy monitoring for the development of HIV cure strategies. An introductory course to ex vivo viral outgrowth assays and its use to quantify the replication competent HIV reservoir will be covered. The participants will receive an intensive training on PCR, qPCR, digital and digital droplet PCR methods with a comprehensive overview of clinical use of these methods and clinical significance of various HIV markers. Novel aspects of HIV persistence such as clonal expansion and integration site will also be discussed and thorough overview of assays to monitor them will be presented. Given that the host’s immune system is an indispensable component of an effective cure strategy, in addition to virological assays, the workshop for the first time will present an overview of immunological assays that are commonly used to assess the potency of both the cellular and humoral immune system. The workshop will conclude with comprehensive hands-on sessions that will include statistical data analysis training sessions.

Target audience

Researchers interested in: low level RNA or DNA quantification by digital PCR and immune monitoring assays especially in the field of HIV research.

Venue

Wednesday and Thursday

Redloft
Hugo Van der Goesstraat 17A, 9000 Ghent

Friday

Ghent University Hosptial, MRBII, ingang 38
De Pintelaan 185, 9000 Ghent
 

Program

Wednesday September 13 2017

 SpeakerInstituteSubject
9:00-09:45Linos VandekerckhoveHIV Cure Research Center UGentIntroduction of HIV-1 reservoir characterisation
9:45-10:30Ward De SpiegelaereGhent UniversityDescription of PCR based methods: PCR, qPCR, digital PCR, droplet digital PCR
10:30-10:50Coffee break
10:50-12:30Maria BuzonVall d'Hebron Institut de Recerca, SpainPractical applications of ex vivo reservoir quantification.
12:30-13:45Lunch
13:45-14:45Sofie RutsaertHIV Cure Research Center UGentClinical applicability and clinical significance of HIV markers (total HIV DNA, 2LTR) and analysis of ddPCR data.
14:45-15:30Sam KintHIV Cure Research Center UGentAssays to assess DNA methylation.
15:30-15:50Coffee break
15:50-16:30Sofie RutsaertHIV Cure Research Center UGentIntegrated HIV DNA quantification.
16:30-17:15Maja KiselinovaUZ GentCell-associated HIV RNA quantification and normalization.

Thursday September 14 2017

 SpeakerInstituteSubject
8:30-9:30Basiel ColeHIV Cure Research Center UGentClonal expansion and Integrated HIV.
9:30-11:00Wim Trypsteen and Clarissa Van HeckeHIV Cure Research Center UGentqPCR and droplet digital PCR data analysis in practice.
11:00-11:20Coffee break
11:20-12:00Magdalena SipsHIV Cure Research Center UGentIntroduction to immune monitoring assays.
12:00-13:15Lunch
13:15-14:15Magdalena SipsHIV Cure Research Center UGentPlasma analytes. Cellular immunity assays. Humoral immunity assays. Histocytometry.
14:15-15:10Pieter PannusInstitute of Tropical Medicine AntwerpCellular immunity part # 1. Viral Inhibition Assay.
15:10-15:30Coffee break
15:30-16:20Philip AdamsLuxembourg Institute of HealthCellular immunity part # 2. Phenotypic and functional flow cytometry-based assays.

Friday September 15 2017

 SpeakerInstituteSubject
8:30-10:30Practical excercies by BioradBioradHand on practice ddPCR platform
10:30-10:45Coffee break
10:45-12:45Practical excercies by BioradBioradHand on practice ddPCR platform
12:45-14:00Lunch