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Workshop 2018-03-05T11:36:32+00:00

HIV Reservoir characterisation Workshop: 14-16 september 2018


Challenges and ethical considerations of HIV cure and treatment interruption trials will be thoroughly discussed during this year’s workshops. Moreover, workshops will provide the participants with a thorough understanding of HIV quantification tools including PCR-based and cell-based functional assays, which are of great importance in therapy monitoring for the development of HIV cure strategies. An introductory course to ex vivo viral outgrowth assays and its use to quantify the replication competent HIV reservoir will be covered. The participants will receive an intensive training on PCR, qPCR, digital and digital droplet PCR methods and single genome sequencing with a comprehensive overview of clinical use of these methods and clinical significance of various HIV markers. Novel aspects of HIV persistence such as clonal expansion and integration site analysis will also be discussed and thorough overview of assays to monitor them will be presented. Given that the host’s immune system is an indispensable component of an effective cure strategy, in addition to virological assays, the workshop will present an overview of immunological assays that are commonly used to assess the competency of the immune system. The workshop will conclude with comprehensive hands-on sessions that will include statistical data analysis training sessions.

Target audience

Researchers interested in: low level RNA or DNA quantification by digital PCR and immune monitoring assays especially in the field of HIV research.

Tentative program

Friday 14 September

08:30-08:40Linos VandekerckhoveHIV Cure Research CenterWelcome
08:40-09:40Ward De SpiegelaereGhent UniversityDescription of PCR based methods: PCR, qPCR, digital PCR, droplet digital PCR
09:40-10:00Coffee break
10:00-12:30Practical exercises dPCRHand on practice dPCR platform
14:00-16:00Practical exercises dPCRHand on practice dPCR platform
16:00-17:00Clarissa Van Hecke and Sofie RutsaertHIV Cure Research CenterqPCR and droplet digital PCR data analysis in practice.
Saturday 15 September

09:00-09:30Linos VandekerckhoveHIV Cure Research Center UGentIntroduction in HIV-1 reservoir characterisation
09:30-10:30Linos Vandekerckhove and Marie-Angelique De ScheerderHIV Cure Research Center UGentHow to set up HIV Cure studies
10:30-10:50Coffee break
10:50-11:50Linos Vandekerckhove and Marie-Angelique De ScheerderHIV Cure Research Center UGentLessons learned from HIV Cure studies
13:30-14:30Sofie RutsaertHIV Cure Research Center UGentClinical applicability and clinical significance of HIV markers (total HIV DNA, 2LTR) and analysis of ddPCR data.
14:30-14:45Coffee break
14:45-15:45Sofie RutsaertHIV Cure Research Center UGentIntegrated HIV DNA quantification.
15:45-16:30Maja KiselinovaUZ GentCell-associated HIV RNA quantification and normalization.
16:30-16:45Coffee break
16:45-17:45Marie-Angelique De ScheerderHIV Cure Research Center UGentSingle Genome sequencing
Sunday 16 September

8:30-9:30Basiel ColeHIV Cure Research Center UGentClonal expansion and Integrated HIV.
9:30-10:30Mathieu AmandInfectious Diseases Research Unit, Luxembourg Institute of HealthHumanized Mice model in HIV research
10:30-11:00Coffee break
11:00-12:00Magdalena SipsHIV Cure Research Center UGentIntroduction to immune monitoring assays.
13:15-14:15Wojciech WitkowskiHIV Cure Research CenterAssessing and improving cellular immunity to HIV.
14:15-14:30Coffee break
14:30-15:30Magdalena SipsHIV Cure Research CenterMonitoring the immune system at the level of cell, tissue and organ.

Practical Information


Registration for the workshop includes registration for the minisymposium on 13 September 2018. It is possible to register from 01/03/2018 until 31/08/2018 (early bird deadline is 30/06/2018). More information can be found here.

The amount of participants is limited to 30 researchers.


Ghent University Hosptial, MRBII, ingang 38
Corneel Heymanslaan 10, 9000 Ghent

HIV reservoir characterisation workshop
HIV Cure Minisymposium